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EMBO Rep. 2012 Dec;13(12):1130-7. doi: 10.1038/embor.2012.161. Epub 2012 Oct 23.

In vivo mutagenesis reveals that OriL is essential for mitochondrial DNA replication.

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1
Department of Medical Biochemistry and Cell Biology, University of Gothenburg, P.O. Box 440, SE-405 30 Gothenburg, Sweden.

Abstract

The mechanisms of mitochondrial DNA replication have been hotly debated for a decade. The strand-displacement model states that lagging-strand DNA synthesis is initiated from the origin of light-strand DNA replication (OriL), whereas the strand-coupled model implies that OriL is dispensable. Mammalian mitochondria cannot be transfected and the requirements of OriL in vivo have therefore not been addressed. We here use in vivo saturation mutagenesis to demonstrate that OriL is essential for mtDNA maintenance in the mouse. Biochemical and bioinformatic analyses show that OriL is functionally conserved in vertebrates. Our findings strongly support the strand-displacement model for mtDNA replication.

PMID:
23090476
PMCID:
PMC3513414
DOI:
10.1038/embor.2012.161
[Indexed for MEDLINE]
Free PMC Article
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