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Acta Crystallogr D Biol Crystallogr. 2012 Nov;68(Pt 11):1584-7. doi: 10.1107/S0907444912038152. Epub 2012 Oct 18.

Nanoflow electrospinning serial femtosecond crystallography.

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1
PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.

Abstract

An electrospun liquid microjet has been developed that delivers protein microcrystal suspensions at flow rates of 0.14-3.1 µl min(-1) to perform serial femtosecond crystallography (SFX) studies with X-ray lasers. Thermolysin microcrystals flowed at 0.17 µl min(-1) and diffracted to beyond 4 Å resolution, producing 14,000 indexable diffraction patterns, or four per second, from 140 µg of protein. Nanoflow electrospinning extends SFX to biological samples that necessitate minimal sample consumption.

PMID:
23090408
PMCID:
PMC3478121
DOI:
10.1107/S0907444912038152
[Indexed for MEDLINE]
Free PMC Article
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