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Exerc Sport Sci Rev. 2013 Apr;41(2):107-15. doi: 10.1097/JES.0b013e3182798a95.

Measuring protein synthesis with SUnSET: a valid alternative to traditional techniques?

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1
Department of Comparative Biosciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI 53706, USA. cgoodman2@wisc.edu

Abstract

Protein synthesis rates commonly are measured using isotopic tracers to quantify the incorporation of a labeled amino acid into muscle proteins. Here we provide evidence supporting our hypothesis that the nonisotopic SUnSET technique is a valid and accurate method for the measurement of in vivo changes in protein synthesis at the whole-muscle and single-muscle fiber levels.

PMID:
23089927
PMCID:
PMC3951011
DOI:
10.1097/JES.0b013e3182798a95
[Indexed for MEDLINE]
Free PMC Article
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