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FEBS Lett. 2012 Nov 30;586(23):4190-6. doi: 10.1016/j.febslet.2012.10.020. Epub 2012 Oct 23.

Sim3 shares some common roles with the histone chaperone Asf1 in fission yeast.

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Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University, Matsue 690-8504, Japan.


An H3/H4 histone chaperone, Asf1, plays an essential role in maintaining genomic stability in many species, including fission yeast. Here, we showed that overexpression of a CENP-A chaperone Sim3 suppressed the temperature sensitive phenotype of asf1-33 and asf1-30 mutants and the defect in chromatin structure, and prevented the accumulation of DNA damage in asf1-33 mutants at high temperatures. Furthermore, asf1-33 and Δsim3 were synthetic lethal. Consistent with this, shutdown of sim3 expression in asf1-33 Δsim3 double mutants that contained extragenic sim3 resulted in growth retardation. In addition, the Δsim3 mutant displayed sensitivity to thiabendazol and hydroxyurea, which suggests that Sim3 plays a general role in maintaining chromatin structure. Our results suggest a possibility that Sim3 functions as a histone chaperone.

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