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Analyst. 2013 Jan 7;138(1):96-9. doi: 10.1039/c2an36186h. Epub 2012 Oct 22.

A novel fluorescence method for determination of pFe3+.

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Institute of Pharmaceutical Science, King's College London, Franklin-Wilkins Building, 150 Stamford Street, London, SE1 9NH, UK.


The fluorescence intensity of probe CP645 in the presence of iron and a competing ligand is associated with pFe(3+) of the competing ligand. A linear correlation was found to exist between the fluorescence and pFe(3+) values. The pFe(3+) values of well studied ligands calculated from the standard curve are consistent with reported values. This novel fluorescence method can be used to determine the pFe(3+) values of ligands which are difficult to measure using conventional spectrophotometric or potentiometric methods.

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