Format

Send to

Choose Destination
See comment in PubMed Commons below
J Biomed Opt. 2012 Sep;17(9):96009-1. doi: 10.1117/1.JBO.17.9.096009.

Infrared microscopic imaging of cutaneous wound healing: lipid conformation in the migrating epithelial tongue.

Author information

1
Rutgers University, Newark College of Arts and Science, Department of Chemistry, 73 Warren Street, Newark, New Jersey 07102, USA.

Abstract

Infrared microscopic imaging has been utilized to analyze for the first time the spatial distribution of lipid structure in an ex vivo human organ culture skin wound healing model. Infrared images were collected at zero, two, four, and six days following wounding. Analysis of lipid infrared spectral properties revealed the presence of a lipid class with disordered chains within and in the vicinity of the migrating epithelial tongue. The presence of lipid ester C=O bands colocalized with the disordered chains provided evidence for the presence of carbonyl-containing lipid species. Gene array data complemented the biophysical studies and provided a biological rationale for the generation of the disordered chain species. This is the first clear observation, to our knowledge, of disordered lipid involvement in cutaneous wound healing. Several possibilities are discussed for the biological relevance of these observations.

PMID:
23085910
DOI:
10.1117/1.JBO.17.9.096009
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Loading ...
    Support Center