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Virology. 2012 Dec 20;434(2):181-6. doi: 10.1016/j.virol.2012.09.036. Epub 2012 Oct 16.

Ocean viruses: rigorously evaluating the metagenomic sample-to-sequence pipeline.

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1
Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, MI, USA.

Abstract

As new environments are studied, viruses consistently emerge as important and prominent players in natural and man-made ecosystems. However, much of what we know is built both upon the foundation of the culturable minority and using methods that are often insufficiently ground-truthed. Here, we review the modern culture-independent viral metagenomic sample-to-sequence pipeline and how next-generation sequencing techniques are drastically altering our ability to systematically and rigorously evaluate them. Together, a series of studies quantitatively evaluate existing and new methods that allow-even for ultra-low DNA samples-the generation of replicable, near-quantitative datasets that maximize inter-comparability and biological inference.

PMID:
23084423
DOI:
10.1016/j.virol.2012.09.036
[Indexed for MEDLINE]
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