Temperature-induced ultradense PEG polyelectrolyte surface grafting provides effective long-term bioresistance against mammalian cells, serum, and whole blood

Biomacromolecules. 2012 Nov 12;13(11):3668-77. doi: 10.1021/bm301125g. Epub 2012 Oct 30.

Abstract

We report a facile method of generating ultradense poly(l-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG) surface by using high temperature alone, which in turn provides dramatic improvement in resisting nonspecific bioadsorption. X-ray photoelectron spectroscopy (XPS) revealed that the surface graft density increased ~4 times higher on the surface prepared at 80 °C compared to 20 °C. The studies from small-angle X-ray scattering (SAXS) and the effect of varying ionic strength during/post assemblies at 20 and 80 °C indicated that the "cloud point grafting effect" is not the cause for obtaining high density grafting. Stringent long-term bioresistance tests have been conducted and the temperature-induced PLL-g-PEG surfaces have achieved (1) zero mammalian cell adsorption/migration for up to 36 days and (2) extremely close-to-zero protein adsorptions have been observed even after 36 days in 10% serum media and 24 h in whole blood within the ultrasensitive detection limit of time-of-flight secondary ion mass spectrometry (ToF-SIMS).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adsorption
  • Animals
  • Blood
  • Cells, Cultured
  • Coated Materials, Biocompatible / chemistry*
  • Humans
  • Osmolar Concentration
  • Photoelectron Spectroscopy
  • Polyethylene Glycols / chemistry*
  • Polylysine / analogs & derivatives*
  • Polylysine / blood
  • Polylysine / chemistry
  • Surface Properties
  • Temperature
  • Transplants*

Substances

  • Coated Materials, Biocompatible
  • polylysine-graft-(poly(ethylene glycol))
  • Polylysine
  • Polyethylene Glycols