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Biochem Biophys Res Commun. 2012 Nov 9;428(1):137-41. doi: 10.1016/j.bbrc.2012.10.026. Epub 2012 Oct 15.

NMR dynamics study of the Z-DNA binding domain of human ADAR1 bound to various DNA duplexes.

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1
Department of Chemistry and RINS, Gyeongsang National University, Jinju, Gyeongnam 660-701, Republic of Korea.

Abstract

The Z-DNA binding domain of human ADAR1 (Zα(ADAR1)) preferentially binds Z-DNA rather than B-DNA with high binding affinity. Here, we have carried out chemical shift perturbation and backbone dynamics studies of Zα(ADAR1) in the free form and in complex with three DNA duplexes, d(CGCGCG)(2), d(CACGTG)(2), and d(CGTACG)(2). This study reveals that Zα(ADAR1) initially binds to d(CGCGCG)(2) through the distinct conformation, especially in the unusually flexible β1-loop-α2 region, from the d(CGCGCG)(2)-(Zα(ADAR1))(2) complex. This study also suggests that Zα(ADAR1) exhibits a distinct conformational change during the B-Z transition of non-CG-repeat DNA duplexes with low binding affinities compared to the CG-repeat DNA duplex.

PMID:
23079620
DOI:
10.1016/j.bbrc.2012.10.026
[Indexed for MEDLINE]
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