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Clin Lab Med. 2012 Dec;32(4):585-99. doi: 10.1016/j.cll.2012.07.005.

Clinical integration of next-generation sequencing technology.

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  • 1Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA.

Abstract

Recent advances in next-generation sequencing (NGS) methods and technology have substantially reduced costs and operational complexity leading to production of benchtop sequencers and commercial software solutions for implementation in small research and clinical laboratories. This article addresses requirements and limitations to successful implementation of these systems, including (1) calibration and validation of the instrumentation, experimental paradigm, and primary readout, (2) secure data transfer, storage, and secondary processing, (3) implementation of software tools for targeted analysis, and (4) training of research and clinical personnel to evaluate data fidelity and interpret the molecular significance of the genomic output.

PMID:
23078661
PMCID:
PMC3479671
DOI:
10.1016/j.cll.2012.07.005
[PubMed - indexed for MEDLINE]
Free PMC Article
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