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Nan Fang Yi Ke Da Xue Xue Bao. 2012 Oct;32(10):1440-4.

[Changes of microRNA expression profiles in Vero cells induced by HSV-2 LAT overexpression].

[Article in Chinese]

Author information

1
Department of Dermatology, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou, China. wangying301@yahoo.com.cn

Abstract

OBJECTIVE:

To investigate the changes in the microRNA expression profile of Vero cells induced by HSV-2 LAT overexpression.

METHODS:

The full-length open reading frame of HSV-2 LAT was synthesized and cloned into pRetroQ- AcGFP1-C1 vector, and the recombinant retrovirus expressing HSV-2 LAT was packaged. Using a microRNA microarray, the microRNA expression profile changes in Vero cells were analyzed after infection with the recombinant retrovirus.

RESULTS:

In Vero cells infected with the recombinant retrovirus for stable HSV-2 LAT overexpression, 5 microRNAs (hsa-miR-23a*, kshv-miR-K12-3, hsa-miR-943, hsa-miR-634, and hsa-miR-1270) were up-regulated and 5 (hsa-miR-181a-2*, hsa-miR-450b-5p, hsa-miR-31, hsa-miR-24, and kshv-miR-K12-12*) were down-regulated.

CONCLUSION:

The expression of HSV-2 LAT can induce changes in microRNA expression profile in Vero cells.

PMID:
23076180
[Indexed for MEDLINE]
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