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Genetics. 1990 Feb;124(2):213-20.

Genetic evidence for two protein domains and a potential new activity in bacteriophage T4 DNA polymerase.

Author information

1
Department of Genetics, University of Alberta, Edmonton, Canada.

Abstract

Intragenic complementation was detected within the bacteriophage T4 DNA polymerase gene. Complementation was observed between specific amino (N)-terminal, temperature-sensitive (ts) mutator mutants and more carboxy (C)-terminal mutants lacking DNA polymerase polymerizing functions. Protein sequences surrounding N-terminal mutation sites are similar to sequences found in Escherichia coli ribonuclease H (RNase H) and in the 5'----3' exonuclease domain of E. coli DNA polymerase I. These observations suggest that T4 DNA polymerase, like E. coli DNA polymerase I, contains a discrete N-terminal domain.

PMID:
2307357
PMCID:
PMC1203915
[Indexed for MEDLINE]
Free PMC Article

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