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Chembiochem. 2012 Nov 5;13(16):2331-4. doi: 10.1002/cbic.201200501. Epub 2012 Oct 15.

Modular assembly of protein building blocks to create precisely defined megamolecules.

Author information

1
Departments of Chemistry and Biomedical Engineering, Howard Hughes Medical Institute, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA.

Abstract

Enzyme-promoted assembly: The construction of a hetero-bifunctional protein building block, HaloTag-cutinase, that reacts rapidly and selectively with a small-molecule linker is described. The step-wise combination of these building blocks generates a 300 kDa "megamolecule" with precisely defined domain orientation, connectivity, and composition.

PMID:
23070998
PMCID:
PMC3804166
DOI:
10.1002/cbic.201200501
[Indexed for MEDLINE]
Free PMC Article

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