Format

Send to

Choose Destination
FEBS Lett. 2012 Nov 16;586(22):4016-22. doi: 10.1016/j.febslet.2012.10.001. Epub 2012 Oct 12.

The E. coli SufS-SufE sulfur transfer system is more resistant to oxidative stress than IscS-IscU.

Author information

1
Department of Chemistry and Biochemistry, University of South Carolina, Columbia, SC 29208, United States.

Abstract

During oxidative stress in Escherichiacoli, the SufABCDSE stress response pathway mediates iron-sulfur (Fe-S) cluster biogenesis rather than the Isc pathway. To determine why the Suf pathway is favored under stress conditions, the stress response SufS-SufE sulfur transfer pathway and the basal housekeeping IscS-IscU pathway were directly compared. We found that SufS-SufE cysteine desulfurase activity is significantly higher than IscS-IscU at physiological cysteine concentrations and after exposure to H(2)O(2). Mass spectrometry analysis demonstrated that IscS-IscU is more susceptible than SufS-SufE to oxidative modification by H(2)O(2). These important results provide biochemical insight into the stress resistance of the Suf pathway.

PMID:
23068614
PMCID:
PMC3511050
DOI:
10.1016/j.febslet.2012.10.001
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Wiley Icon for PubMed Central
Loading ...
Support Center