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FEBS Lett. 2012 Nov 16;586(22):3962-8. doi: 10.1016/j.febslet.2012.09.045. Epub 2012 Oct 12.

NDRG2 and PRA1 interact and synergistically inhibit T-cell factor/β-catenin signaling.

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1
Medical Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea.

Abstract

NDRG2 is a member of the N-myc downstream regulated gene (NDRG) family, implicated in cell growth and differentiation. Investigation of NDRG2 molecular interactions by yeast two-hybrid screening identified prenylated Rab acceptor-1 (PRA1), involved in vesicle trafficking and protein transport, as binding partner. Binding of NDRG2 (and NDRG1-4) with PRA1 in vitro was confirmed by GST pull-down assay and immunoprecipitation, and colocalization was verified by confocal microscopy in HCT116 cells. Intracellular coexpression showed that NDRG2 and PRA1 synergistically downregulate T-cell factor (TCF) promoter activity and GSK3β phosphorylation. Results suggest that NDRG2 and PRA1 might act synergistically to prevent signaling of TCF/β-catenin.

PMID:
23068607
DOI:
10.1016/j.febslet.2012.09.045
[Indexed for MEDLINE]
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