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Vaccine. 2012 Dec 7;30(51):7368-73. doi: 10.1016/j.vaccine.2012.09.082. Epub 2012 Oct 10.

Distribution of surface glycoproteins on influenza A virus determined by electron cryotomography.

Author information

1
Division of Physical Biochemistry, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London, NW7 1AA, United Kingdom.

Abstract

We use electron cryotomography to reconstruct virions of two influenza A H3N2 virus strains. The maps reveal the structure of the viral envelope containing hemagglutinin (HA) and neuraminidase (NA) glycoproteins and the virus interior containing a matrix layer and an assembly of ribonucleoprotein particles (RNPs) that package the genome. We build a structural model for the viral surface by locating copies of the X-ray structure of the HA ectodomain into density peaks on the virus surface. We calculate inter-glycoprotein distances and the fractional volume occupied by glycoproteins. The models suggest that for typical HA densities on virus, Fabs can bind to epitopes on the HA stem domain. The models also show how membrane curvature may influence the number of glycoproteins that can simultaneously interact with a target surface of receptors.

PMID:
23063838
PMCID:
PMC3532595
DOI:
10.1016/j.vaccine.2012.09.082
[Indexed for MEDLINE]
Free PMC Article

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