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Mol Brain. 2012 Oct 12;5:38. doi: 10.1186/1756-6606-5-38.

Protease activated receptor 1-induced glutamate release in cultured astrocytes is mediated by Bestrophin-1 channel but not by vesicular exocytosis.

Author information

1
Korea Institute of Science and Technology, Seoul, South Korea.

Abstract

BACKGROUND:

Glutamate is the major transmitter that mediates the principal form of excitatory synaptic transmission in the brain. It has been well established that glutamate is released via Ca2+-dependent exocytosis of glutamate-containing vesicles in neurons. However, whether astrocytes exocytose to release glutamate under physiological condition is still unclear.

FINDINGS:

We report a novel form of glutamate release in astrocytes via the recently characterized Ca2+-activated anion channel, Bestrophin-1 (Best1) by Ca2+ dependent mechanism through the channel pore. We demonstrate that upon activation of protease activated receptor 1 (PAR1), an increase in intracellular Ca2+ concentration leads to an opening of Best1 channels and subsequent release of glutamate in cultured astrocytes.

CONCLUSIONS:

These results provide strong molecular evidence for potential astrocyte-neuron interaction via Best1-mediated glutamate release.

PMID:
23062602
PMCID:
PMC3539998
DOI:
10.1186/1756-6606-5-38
[Indexed for MEDLINE]
Free PMC Article

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