Format

Send to

Choose Destination
See comment in PubMed Commons below
Anal Chem. 2012 Nov 6;84(21):9609-14. doi: 10.1021/ac3025703. Epub 2012 Oct 23.

Miniaturized electroosmotic pump capable of generating pressures of more than 1200 bar.

Author information

1
Department of Chemistry and Biochemistry, University of Oklahoma, Norman, Oklahoma 73019, United States.

Abstract

The pressure output of a pump cannot be increased simply by connecting several of them in series. This barrier is eliminated with the micropump developed in this work. The pump is actually an assembly of a number of fundamental pump units connected in series. The maximum pressure output of this pump assembly is directly proportional to the number of serially connected pump units. Theoretically, one can always enhance the pressure output by adding more pump units in the assembly, but in reality the upper pressure is constrained by the microtees or microunions joining the pump components. With commercially available microtees and microunions, pressures of more than 1200 bar have been achieved. We have recently experimented using open capillaries to build this pump, but many capillaries have to be utilized in parallel to produce an adequate flow to drive HPLC separations. In this paper, we synthesize polymer monoliths inside 75 μm i.d. capillaries, use these monoliths to assemble miniaturized pumps, characterize the performance of these pumps, and employ these pumps for HPLC separations of intact proteins. By tuning the experimental parameters for monolith preparations, we obtain both negatively and positively charged submicrometer capillary channels conveniently. Each monolith in a 75 μm i.d. capillary is equivalent to several thousands of open capillaries.

PMID:
23061696
DOI:
10.1021/ac3025703
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for American Chemical Society
    Loading ...
    Support Center