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IEEE J Biomed Health Inform. 2013 Jan;17(1):232-9. doi: 10.1109/TITB.2012.2222654. Epub 2012 Oct 4.

Classification of bacterial contamination using image processing and distributed computing.

Abstract

Disease outbreaks due to contaminated food are a major concern not only for the food-processing industry but also for the public at large. Techniques for automated detection and classification of microorganisms can be a great help in preventing outbreaks and maintaining the safety of the nations food supply. Identification and classification of foodborne pathogens using colony scatter patterns is a promising new label-free technique that utilizes image-analysis and machine-learning tools. However, the feature-extraction tools employed for this approach are computationally complex, and choosing the right combination of scatter-related features requires extensive testing with different feature combinations. In the presented work we used computer clusters to speed up the feature-extraction process, which enables us to analyze the contribution of different scatter-based features to the overall classification accuracy. A set of 1000 scatter patterns representing ten different bacterial strains was used. Zernike and Chebyshev moments as well as Haralick texture features were computed from the available light-scatter patterns. The most promising features were first selected using Fishers discriminant analysis, and subsequently a support-vector-machine (SVM) classifier with a linear kernel was used. With extensive testing we were able to identify a small subset of features that produced the desired results in terms of classification accuracy and execution speed. The use of distributed computing for scatter-pattern analysis, feature extraction, and selection provides a feasible mechanism for large-scale deployment of a light scatter-based approach to bacterial classification.

PMID:
23060342
DOI:
10.1109/TITB.2012.2222654
[Indexed for MEDLINE]

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