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Angew Chem Int Ed Engl. 2012 Dec 7;51(50):12519-23. doi: 10.1002/anie.201206749. Epub 2012 Oct 10.

In Situ probing of newly synthesized peptidoglycan in live bacteria with fluorescent D-amino acids.

Author information

1
Indiana University, Bloomington, IN 47405, USA.

Abstract

Tracking a bug's life: Peptidoglycan (PG) of diverse bacteria is labeled by exploiting the tolerance of cells for incorporating different non-natural D-amino acids. These nontoxic D-amino acids preferably label the sites of active PG synthesis, thereby enabling fine spatiotemporal tracking of cell-wall dynamics in phylogenetically and morphologically diverse bacteria. HCC = 7-hydroxycoumarin, NBD = 7-nitrobenzofurazan, TAMRA = carboxytetramethylrhodamine.

PMID:
23055266
PMCID:
PMC3589519
DOI:
10.1002/anie.201206749
[Indexed for MEDLINE]
Free PMC Article

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