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J Clin Microbiol. 2012 Dec;50(12):4144-6. doi: 10.1128/JCM.02084-12. Epub 2012 Oct 10.

Allele-specific PCR for determination of IL28B genotype.

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  • 1Department of Laboratory Medicine, University of Washington, Seattle, Washington, USA.

Abstract

The IL28B genotype is a critical determinant of interferon response in patients infected with hepatitis C virus genotype 1. We describe an allele-specific PCR assay for the IL28B genotype. The assay is simple and robust, uses commonly available real-time PCR instrumentation, and is well suited for clinical laboratories offering IL28B genotyping.

PMID:
23052312
PMCID:
PMC3502971
DOI:
10.1128/JCM.02084-12
[PubMed - indexed for MEDLINE]
Free PMC Article
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