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N Am J Med Sci. 2012 Sep;4(9):429-34. doi: 10.4103/1947-2714.100998.

Western blot: technique, theory, and trouble shooting.

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1
Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada.

Abstract

Western blotting is an important technique used in cell and molecular biology. By using a western blot, researchers are able to identify specific proteins from a complex mixture of proteins extracted from cells. The technique uses three elements to accomplish this task: (1) separation by size, (2) transfer to a solid support, and (3) marking target protein using a proper primary and secondary antibody to visualize. This paper will attempt to explain the technique and theory behind western blot, and offer some ways to troubleshoot.

KEYWORDS:

Bio-medical research; protein; western blot

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