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G3 (Bethesda). 2012 Oct;2(10):1213-21. doi: 10.1534/g3.112.003046. Epub 2012 Oct 1.

Global gene expression in Coprinopsis cinerea meiotic mutants reflects checkpoint arrest.

Author information

1
Biology Department, Indiana University, Bloomington, Indiana 47405, USA.

Abstract

The basidiomycete Coprinopsis cinerea is well-suited to studies of meiosis because meiosis progresses synchronously in 10 million cells within each mushroom cap. Approximately 20% of C. cinerea genes exhibit changing expression during meiosis, but meiosis and mushroom development happen concurrently and therefore differentially expressed genes might not be directly involved in meiotic processes. By using microarrays, we examined global gene expression across a meiotic time course in two mutants in which meiosis arrests but mushrooms develop normally. Genes differentially expressed in the mutants compared with the wild type are likely to be involved in meiosis and sporulation as opposed to mushroom development. In rad50-1, which arrests in late prophase, RNA abundance for a group of early meiotic genes remains high, whereas the expression of a group of late meiotic genes is never induced. In contrast, in msh5-22 (which fails to undergo premeiotic DNA replication), both early and late meiotic genes are underexpressed relative to wild type at late meiotic time points as the cells die. Genes that are differentially expressed relative to wild type in both mutants are particularly strong candidates for playing roles in meiosis and sporulation.

KEYWORDS:

MRN complex; checkpoint arrest; meiotic S phase; microarray analysis; mushroom development

PMID:
23050232
PMCID:
PMC3464114
DOI:
10.1534/g3.112.003046
[Indexed for MEDLINE]
Free PMC Article

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