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Nat Commun. 2012;3:1112. doi: 10.1038/ncomms2112.

A multi-omic map of the lipid-producing yeast Rhodosporidium toruloides.

Author information

1
Division of Biotechnology, Dalian Institute of Chemical Physics, CAS, 457 Zhongshan Road, Dalian 116023, China.

Erratum in

  • Nat Commun. 2013;4:1930. Zou, Hanfan [corrected to Zou, Hanfa].

Abstract

Triacylglycerols are among the most attractive alternative raw materials for biofuel development. Current oil plant-based technologies are limited in terms of triacylglycerol production capacity and rate. These limitations may be circumvented by biotransformation of carbohydrates into lipids; however, our understanding of microbial oleaginicity remains limited. Here we present the results of a multi-omic analysis of Rhodosporidium toruloides, a robust triacylglycerol-producing fungus. The assembly of genome and transcriptome sequencing data reveals a genome of 20.2 Mb containing 8,171 protein-coding genes, the majority of which have multiple introns. Genes including a novel fatty acid synthase are predicted to participate in metabolic pathways absent in non-oleaginous yeasts. Transcriptomic and proteomic data suggest that lipid accumulation under nitrogen-limited conditions correlates with the induction of lipogenesis, nitrogenous compound recycling, macromolecule metabolism and autophagy. The multi-omic map of R. toruloides therefore provides a valuable resource for efforts to rationally engineer lipid-production pathways.

PMID:
23047670
PMCID:
PMC3493640
DOI:
10.1038/ncomms2112
[Indexed for MEDLINE]
Free PMC Article
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