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Biochim Biophys Acta. 2013 Jan;1830(1):2256-66. doi: 10.1016/j.bbagen.2012.10.001. Epub 2012 Oct 6.

Cellular internalization and stress response of ingested amorphous silica nanoparticles in the midgut of Drosophila melanogaster.

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1
CSIR-Indian Institute of Toxicology Research, Uttar Pradesh, India.

Abstract

BACKGROUND:

Amorphous silica nanoparticles (aSNPs) are used for various applications including food industry. However, limited in vivo studies are available on absorption/internalization of ingested aSNPs in the midgut cells of an organism. The study aims to examine cellular uptake of aSNPs (<30nm) in the midgut of Drosophila melanogaster (Oregon R(+)) owing to similarities between the midgut tissue of this organism and human and subsequently cellular stress response generated by these nanoparticles.

METHODS:

Third instar larvae of D. melanogaster were exposed orally to 1-100μg/mL of aSNPs for 12-36h and oxidative stress (OS), heat shock genes (hsgs), membrane destabilization (Acridine orange/Ethidium Bromide staining), cellular internalization (TEM) and apoptosis endpoints.

RESULTS:

A significant increase was observed in OS endpoints in the midgut cells of exposed Drosophila in a concentration- and time-dependent manner. Significantly increased expression of hsp70 and hsp22 along with caspases activation, membrane destabilization and mitochondrial membrane potential loss was also observed. TEM analysis showed aSNPs-uptake in the midgut cells of exposed Drosophila via endocytic vesicles and by direct membrane penetration.

CONCLUSION:

aSNPs after their internalization in the midgut cells of exposed Drosophila larvae show membrane destabilization along with increased cellular stress and cell death.

GENERAL SIGNIFICANCE:

Ingested aSNPs show adverse effects on the cells of GI tract of the exposed organism thus their industrial use as a food-additive may raise concern to human health.

PMID:
23046978
DOI:
10.1016/j.bbagen.2012.10.001
[Indexed for MEDLINE]
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