Format

Send to

Choose Destination
See comment in PubMed Commons below
Transfusion. 2013 Jun;53(6):1201-4. doi: 10.1111/j.1537-2995.2012.03918.x. Epub 2012 Oct 9.

Measurement of the absolute immature platelet number reflects marrow production and is not impacted by platelet transfusion.

Author information

1
Hematology Branch, National Heart, Lung, and BloodInstitute, National Institutes of Health, Bethesda, MD 20892, USA.

Abstract

BACKGROUND:

The ability to distinguish increased platelet (PLT) destruction from PLT hypoproduction is important in the care of patients with marrow failure syndromes and patients receiving high-dose chemotherapy. The measurement of immature circulating PLTs based on RNA content using an automated counter is now feasible. This study evaluated the impact of recent PLT transfusion on measurement of immature PLT variables.

STUDY DESIGN AND METHODS:

The immature PLT fraction (IPF) and absolute immature PLT number (AIPN) were measured using a hematology analyzer before and after PLT transfusion in nine transfusion-dependent patients with marrow failure secondary to aplastic anemia, myelodysplasia, or transplantation conditioning. IPF and AIPN were also measured serially over 5 days of storage in three plateletpheresis components collected from normal donors.

RESULTS:

PLT transfusion did not significantly change the mean AIPN in transfused patients. In contrast, IPF decreased significantly from 6.6 ± 4.6% on Day -1 to 2.3 ± 1.4% on Day 0 before returning to 4.3 ± 2.3% on Day +1. In the PLT component, AIPN and IPF% increased significantly over 5 days of storage, most likely due to an artifact of the staining and detection process for stored PLTs, no longer detected in vivo once the PLTs were transfused.

CONCLUSION:

PLT transfusion decreases the IPF due to the resultant increase in circulating PLT count. However, PLT transfusion does not change the circulating AIPN, validating this assay as a reflection of ongoing PLT production by the marrow in various clinical settings, regardless of proximity to PLT transfusion.

PMID:
23043309
PMCID:
PMC3543490
DOI:
10.1111/j.1537-2995.2012.03918.x
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Wiley Icon for PubMed Central
    Loading ...
    Support Center