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Nat Methods. 2012 Nov;9(11):1077-80. doi: 10.1038/nmeth.2205. Epub 2012 Oct 7.

Iterative plug-and-play methodology for constructing and modifying synthetic gene networks.

Author information

1
Howard Hughes Medical Institute, Boston University, Boston, Massachusetts, USA.

Abstract

We present a methodology for the design, construction and modification of synthetic gene networks. This method emphasizes post-assembly modification of constructs based on network behavior, thus facilitating iterative design strategies and rapid tuning and repurposing of gene networks. The ease of post-construction modification afforded by this approach and the ever-increasing repository of components within the framework will help accelerate the development of functional genetic circuits for synthetic biology.

PMID:
23042452
PMCID:
PMC3492501
DOI:
10.1038/nmeth.2205
[Indexed for MEDLINE]
Free PMC Article

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