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Nat Chem Biol. 2012 Nov;8(11):926-32. doi: 10.1038/nchembio.1087. Epub 2012 Oct 7.

A RubisCO-like protein links SAM metabolism with isoprenoid biosynthesis.

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1
Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Champaign, IL, USA.

Abstract

Functional assignment of uncharacterized proteins is a challenge in the era of large-scale genome sequencing. Here, we combine in extracto NMR, proteomics and transcriptomics with a newly developed (knock-out) metabolomics platform to determine a potential physiological role for a ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO)-like protein from Rhodospirillum rubrum. Our studies unraveled an unexpected link in bacterial central carbon metabolism between S-adenosylmethionine-dependent polyamine metabolism and isoprenoid biosynthesis and also provide an alternative approach to assign enzyme function at the organismic level.

PMID:
23042035
PMCID:
PMC3475740
DOI:
10.1038/nchembio.1087
[Indexed for MEDLINE]
Free PMC Article
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