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J Microbiol Methods. 2012 Dec;91(3):520-6. doi: 10.1016/j.mimet.2012.09.031. Epub 2012 Oct 2.

OEM--a new medium for rapid isolation of onion-pathogenic and onion-associated bacteria.

Author information

1
Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, USA. amz44@cornell.edu

Abstract

Onions (Allium cepa L.) are plagued by a number of bacterial pathogens including Pantoea ananatis, P. agglomerans, Burkholderia cepacia, Enterobacter cloacae, Pectobacterium carotovorum subsp. carotovorum, Xanthomonas axonopodis pv. axonopodis and several Pseudomonas spp. We developed a semi-selective medium, termed onion extract medium (OEM), to selectively and rapidly isolate bacteria pathogenic to and associated with onions and onion-related samples including bulbs, seeds, sets, transplant seedlings, soil and water. Most strains of interest grow sufficiently on OEM in 24h at 28°C for tentative identification based on colony morphology, facilitating further characterization by microbiological and/or molecular means.

PMID:
23041494
DOI:
10.1016/j.mimet.2012.09.031
[Indexed for MEDLINE]

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