Primer design for identifying economically important Liriomyza species (Diptera: Agromyzidae) by multiplex PCR

Mol Ecol Resour. 2013 Jan;13(1):96-102. doi: 10.1111/1755-0998.12025. Epub 2012 Oct 8.

Abstract

Leafminer flies, especially, Liriomyza huidobrensis, Liriomyza sativae and Liriomyza trifolii, are quarantine species in many countries. Their morphological similarity makes identification difficult. To develop a rapid, reliable, sensitive and simple molecular identification method using multiplex PCR, we newly sequenced the mitochondrial cytochrome oxidase I (COI) genes of Liriomyza bryoniae, Liriomyza chinensis, L. huidobrensis, L. sativae, L. trifolii, Chromatomyia horticola and four parasitoid species. We aligned them with all the COI sequences of the leafminer flies found in the international DNA nucleotide sequence databases (DDBJ/EMBL/GenBank). We then designed species-specific primers to allow us to differentiate between L. bryoniae, L. chinensis, L. huidobrensis, L. sativae, and L. trifolii.

MeSH terms

  • Animals
  • Base Sequence
  • Computational Biology
  • DNA Primers / genetics*
  • Diptera / genetics*
  • Electron Transport Complex IV / genetics
  • Electrophoresis, Agar Gel
  • Molecular Sequence Data
  • Multiplex Polymerase Chain Reaction / methods*
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Species Specificity

Substances

  • DNA Primers
  • Electron Transport Complex IV