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Opt Express. 2012 Sep 24;20(20):22245-51. doi: 10.1364/OE.20.022245.

A novel method of diffraction imaging flow cytometry for sizing microspheres.

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1
Department of Biomedical Engineering, Tianjin University, Tianjin 300072, China.

Abstract

We report a novel method of diffraction imaging flow cytometry to measure and analyze size distribution of microspheres. An automated and robust image processing software based on the short-time-Fourier-transform algorithm has been developed to analyze the characteristic and spatially varying oscillations of side scatters recorded as a diffraction image. Our results demonstrate that the new method allows accurate and rapid determination of single microspheres' diameters ranging from 1 to 100 μm. The capacity for analysis of light scattering by two-sphere aggregates has been demonstrated but analytical tools for characterization of aggregates by multiple microspheres remain to be developed.

PMID:
23037372
[Indexed for MEDLINE]
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