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Methods. 2013 Feb;59(2):217-24. doi: 10.1016/j.ymeth.2012.09.009. Epub 2012 Oct 2.

A genome wide RNA interference screening method to identify host factors that modulate influenza A virus replication.

Author information

1
Microbiology and Physiological Systems Department, University of Massachusetts Medical School, Worcester, MA 01655, USA.

Abstract

The use of genome wide RNA interference (RNAi) screens to investigate host-virals interactions has revealed unexpected connections that have improved our understanding of viral pathogenesis and cell biology. This work describes the use of an RNAi screening method employing an immunofluorescence image-based strategy and influenza A virus. We find this approach to be readily implemented, scalable and amenable to the direct evaluation of a variety of viral lifecycles.

PMID:
23036328
PMCID:
PMC4145670
DOI:
10.1016/j.ymeth.2012.09.009
[Indexed for MEDLINE]
Free PMC Article

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