Format

Send to

Choose Destination
See comment in PubMed Commons below
Methods. 2013 Feb;59(2):217-24. doi: 10.1016/j.ymeth.2012.09.009. Epub 2012 Oct 2.

A genome wide RNA interference screening method to identify host factors that modulate influenza A virus replication.

Author information

  • 1Microbiology and Physiological Systems Department, University of Massachusetts Medical School, Worcester, MA 01655, USA.

Abstract

The use of genome wide RNA interference (RNAi) screens to investigate host-virals interactions has revealed unexpected connections that have improved our understanding of viral pathogenesis and cell biology. This work describes the use of an RNAi screening method employing an immunofluorescence image-based strategy and influenza A virus. We find this approach to be readily implemented, scalable and amenable to the direct evaluation of a variety of viral lifecycles.

PMID:
23036328
PMCID:
PMC4145670
DOI:
10.1016/j.ymeth.2012.09.009
[PubMed - indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science Icon for PubMed Central
    Loading ...
    Support Center