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Mol Ther. 2013 Jan;21(1):185-91. doi: 10.1038/mt.2012.180. Epub 2012 Oct 2.

Systemically injected exosomes targeted to EGFR deliver antitumor microRNA to breast cancer cells.

Author information

1
Department of Molecular Pathology, Tokyo Medical University, Tokyo, Japan.

Abstract

Despite the therapeutic potential of nucleic acid drugs, their clinical application has been limited in part by a lack of appropriate delivery systems. Exosomes or microvesicles are small endosomally derived vesicles that are secreted by a variety of cell types and tissues. Here, we show that exosomes can efficiently deliver microRNA (miRNA) to epidermal growth factor receptor (EGFR)-expressing breast cancer cells. Targeting was achieved by engineering the donor cells to express the transmembrane domain of platelet-derived growth factor receptor fused to the GE11 peptide. Intravenously injected exosomes delivered let-7a miRNA to EGFR-expressing xenograft breast cancer tissue in RAG2(-/-) mice. Our results suggest that exosomes can be used therapeutically to target EGFR-expressing cancerous tissues with nucleic acid drugs.

PMID:
23032975
PMCID:
PMC3538304
DOI:
10.1038/mt.2012.180
[Indexed for MEDLINE]
Free PMC Article

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