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Biol Chem. 2012 Apr;393(4):249-58. doi: 10.1515/hsz-2011-0245.

N-glycoproteomics: mass spectrometry-based glycosylation site annotation.

Author information

1
Department of Bioanalytics, Leibniz-Institut für Analytische Wissenschaften, ISAS eV, D-44227 Dortmund, Germany.

Abstract

Glycosylations are ubiquitous and, in many cases, essential protein modifications. Yet comprehensive and detailed analysis of glycosylations on a proteome-wide scale is a daunting and still unsolved challenge. However, a common workflow has emerged over the last decade for large-scale N-glycosylation site annotation by application of proteomic methodology. Thereby, the qualitative and quantitative assessment of hundreds or thousands of modification sites is enabled. This review presents a short overview about common enrichment techniques and glycosylation site detection for N-glycopeptides, including benefits and challenges of analysis.

PMID:
23029648
DOI:
10.1515/hsz-2011-0245
[Indexed for MEDLINE]

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