Format

Send to

Choose Destination
PLoS One. 2012;7(9):e46140. doi: 10.1371/journal.pone.0046140. Epub 2012 Sep 27.

Profiling the anaerobic response of C. elegans using GC-MS.

Author information

1
The Barshop Institute for Longevity and Aging Studies, University of Texas Health Science Center at San Antonio, United States of America.

Abstract

The nematode Caenorhabditis elegans is a model organism that has seen extensive use over the last four decades in multiple areas of investigation. In this study we explore the response of the nematode Caenorhabditis elegans to acute anoxia using gas-chromatography mass-spectrometry (GC-MS). We focus on the readily-accessible worm exometabolome to show that C. elegans are mixed acid fermenters that utilize several metabolic pathways in unconventional ways to remove reducing equivalents - including partial reversal of branched-chain amino acid catabolism and a potentially novel use of the glyoxylate pathway. In doing so, we provide detailed methods for the collection and analysis of excreted metabolites that, with minimal adjustment, should be applicable to many other species. We also describe a procedure for collecting highly volatile compounds from C. elegans. We are distributing our mass spectral library in an effort to facilitate wider use of metabolomics.

PMID:
23029411
PMCID:
PMC3459875
DOI:
10.1371/journal.pone.0046140
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Public Library of Science Icon for PubMed Central
Loading ...
Support Center