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PLoS One. 2012;7(9):e44752. doi: 10.1371/journal.pone.0044752. Epub 2012 Sep 21.

MR angiography, MR imaging and proton MR spectroscopy in-vivo assessment of skeletal muscle ischemia in diabetic rats.

Author information

1
Department of Neuroscience and Imaging, Institute for Advanced Biomedical Technologies, G. d'Annunzio University Foundation, Chieti, Italy.

Abstract

To prospectively evaluate the feasibility of using magnetic resonance (MR) techniques for in-vivo assessing a rat diabetic model of limb ischemia. Unilateral hind limb ischemia was induced by ligation of the iliac-femoral artery in male streptozotocin-treated and non-diabetic control rats. Four weeks after ligation, rats underwent MR Angiography (MRA), T(1)-weighted and Short Time Inversion Recovery (STIR) sequences and muscle Proton MR Spectroscopy ((1)H-MRS) on both hind limbs. After MR examinations, immunoblotting and immunofluorescence analysis were performed. MRA showed a signal void due to flow discontinuation distal to the artery ligation. T(1)-weighted and STIR images showed, respectively, the presence of tissue swelling (p = 0.018 for non-diabetic; p = 0.027 for diabetic rats) and signal hyperintensity in tissue affected by occlusion. Mean total creatine/water for the occluded limb was significantly lower than for the non-occluded limbs in both non-diabetic (5.46×10(-4) vs 1.14×10(-3), p = 0.028) and diabetic rats (1.37×10(-4) vs 1.10×10(-3); p = 0.018). MR Imaging and (1)H-MRS changes were more pronounced in diabetic than in non-diabetic occluded limbs (p = 0.032). MR findings were confirmed by using histological findings. Combined MR techniques can be used to demonstrate the presence of structural and metabolic changes produced by iliac-femoral artery occlusion in rat diabetic model of limb ischemia.

PMID:
23028603
PMCID:
PMC3448608
DOI:
10.1371/journal.pone.0044752
[Indexed for MEDLINE]
Free PMC Article

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