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Opt Lett. 2012 Oct 1;37(19):4062-4. doi: 10.1364/OL.37.004062.

Quantitative phase imaging using a partitioned detection aperture.

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1
Boston University, Department of Biomedical Engineering, 44 Cummington Street, Boston, Massachusetts 02215, USA.

Abstract

We present a technique to quantitatively image the phase of thin quasi-transparent samples using extended source incoherent illumination and off-axis detection apertures. Our technique is achromatic and polarization independent, requires no active elements, and can be readily adapted to standard bright-field microscopes. We demonstrate our technique by quantitatively reconstructing the phase of cheek cells and a microlens. The light efficient, single-shot nature of our technique enables phase imaging at frame rates that are camera limited.

PMID:
23027279
[Indexed for MEDLINE]
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