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Dent Mater. 2012 Dec;28(12):1250-60. doi: 10.1016/j.dental.2012.09.002. Epub 2012 Sep 29.

Micropatterned silica thin films with nanohydroxyapatite micro-aggregates for guided tissue regeneration.

Author information

1
INEB - Instituto de Engenharia Biomédica, Universidade do Porto, Rua Campo Alegre, 823, 4150-180 Porto, Portugal. angela.carvalho@ineb.up.pt

Abstract

Surface modification of biomaterials has been shown to improve the biological response to dental implants. The ability to create a controlled micro-texture on the implant via additive surface modification techniques with bioactive nanohydroxyapatite (nanoHA) may positively influence guided tissue regeneration.

OBJECTIVE:

The main goal of this study was to produce micro-fabricated SiO(2) surfaces modified with nanohydroxyapatite particles and to characterize their influence on the biological response of Human Dental-Pulp Mesenchymal Stem Cells (hDP-MSCs) and Streptococcus mutans.

MATERIALS AND METHODS:

A combined methodology of sol-gel and soft-lithography was used to produce micropatterned SiO(2) thin films with different percentages of nanoHA micro-aggregates. The surfaces were characterized by SEM/EDS, FT-IR/ATR, AFM, XPS quantitative elemental percentage and contact angle measurements. Biological characterization was performed using hDP-MSCs cultures, while Streptococcus mutans was the selected microorganism to evaluate the bacterial adhesion on the thin films.

RESULTS:

Micropatterned SiO(2) surfaces with 0%, 1% and 5% of nanoHA micro-aggregates were successfully produced using a combination of sol-gel and soft-lithography. These surfaces controlled the biological response, triggering alignment and oriented proliferation of hDP-MSCs and significant differences in the adhesion of S. mutans to the different surfaces.

SIGNIFICANCE:

The micropatterned surfaces exhibited biocompatible behavior that induced an oriented adhesion and proliferation of hDP-MSCs while SiO(2) presented low bacterial adhesion. These results show that the combination of sol-gel with soft-lithography is a good approach to create micropatterned surfaces with bioactive nanoparticles for guided tissue regeneration.

PMID:
23026648
DOI:
10.1016/j.dental.2012.09.002
[Indexed for MEDLINE]

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