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Nat Immunol. 2012 Nov;13(11):1118-28. doi: 10.1038/ni.2419. Epub 2012 Sep 30.

Gene-expression profiles and transcriptional regulatory pathways that underlie the identity and diversity of mouse tissue macrophages.

Author information

1
Department of Developmental and Regenerative Biology, Mount Sinai School of Medicine, New York, New York, USA.

Abstract

We assessed gene expression in tissue macrophages from various mouse organs. The diversity in gene expression among different populations of macrophages was considerable. Only a few hundred mRNA transcripts were selectively expressed by macrophages rather than dendritic cells, and many of these were not present in all macrophages. Nonetheless, well-characterized surface markers, including MerTK and FcγR1 (CD64), along with a cluster of previously unidentified transcripts, were distinctly and universally associated with mature tissue macrophages. TCEF3, C/EBP-α, Bach1 and CREG-1 were among the transcriptional regulators predicted to regulate these core macrophage-associated genes. The mRNA encoding other transcription factors, such as Gata6, was associated with single macrophage populations. We further identified how these transcripts and the proteins they encode facilitated distinguishing macrophages from dendritic cells.

PMID:
23023392
PMCID:
PMC3558276
DOI:
10.1038/ni.2419
[Indexed for MEDLINE]
Free PMC Article

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