Abstract
Six commercially available DNA extraction kits, as well as thermal lysis and proteinase K DNA extraction were evaluated regarding bacterial inactivation, DNA yield and purity, and their use in a Burkholderia pseudomallei real-time PCR. While all methods successfully inactivated the bacteria, by measuring DNA purity and the level of detection by real-time PCR, the proteinase K method was the most sensitive.
Copyright © 2012 Elsevier B.V. All rights reserved.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Analytic Sample Preparation Methods / methods*
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Burkholderia pseudomallei / chemistry*
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Burkholderia pseudomallei / genetics*
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Burkholderia pseudomallei / isolation & purification
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DNA, Bacterial / genetics
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DNA, Bacterial / isolation & purification*
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Endopeptidase K / chemistry
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Hot Temperature
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Reagent Kits, Diagnostic / economics
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Real-Time Polymerase Chain Reaction
Substances
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DNA, Bacterial
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Reagent Kits, Diagnostic
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Endopeptidase K