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Biochem Biophys Res Commun. 1990 Jan 15;166(1):43-9.

Cloning of a hsp70-related gene expressed in mouse spermatids.

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Laboratory of Cell Biology, Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.


A cDNA library of spermatids was screened by a differential hybridization in order to isolate genes expressed in haploid cells of the mouse male germ line. A clone was found that encoded a protein related to the heat shock protein 70. A genomic DNA clone comparable to this cDNA clone was also isolated from a mouse genomic library. This gene had only one continuous open reading frame capable of coding a 630 amino-acid protein. There was an excellent match of the sequence with the heat shock protein 70 family but a difference from any previous 70 kilodalton heat shock protein. A 2.7kb transcript derived from this gene was expressed in spermatids but not in other testicular germ cells and somatic tissues. We have referred to this gene as hsc70t.

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