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N Engl J Med. 2012 Sep 27;367(13):1220-7.

Use of reprogrammed cells to identify therapy for respiratory papillomatosis.

Author information

1
Department of Pathology, Georgetown University Medical Center, Washington, DC 20057, USA.

Abstract

A patient with a 20-year history of recurrent respiratory papillomatosis had progressive, bilateral tumor invasion of the lung parenchyma. We used conditional reprogramming to generate cell cultures from the patient's normal and tumorous lung tissue. Analysis revealed that the laryngeal tumor cells contained a wild-type 7.9-kb human papillomavirus virus type 11 (HPV-11) genome, whereas the pulmonary tumor cells contained a 10.4-kb genome. The increased size of the latter viral genome was due to duplication of the promoter and oncogene regions. Chemosensitivity testing identified vorinostat as a potential therapeutic agent. At 3 months after treatment initiation, tumor sizes had stabilized, with durable effects at 15 months.

PMID:
23013073
PMCID:
PMC4030597
DOI:
10.1056/NEJMoa1203055
[Indexed for MEDLINE]
Free PMC Article

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