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J Clin Endocrinol Metab. 2012 Dec;97(12):4613-22. doi: 10.1210/jc.2012-2098. Epub 2012 Sep 25.

Stimulation of GPR30 increases release of EMMPRIN-containing microvesicles in human uterine epithelial cells.

Author information

1
Department of Animal Sciences, University of Illinois, Urbana, Illinois, 61801, USA. lburne3@illinois.edu

Erratum in

  • J Clin Endocrinol Metab. 2013 Nov;98(11):4546.

Abstract

CONTEXT:

Uterine remodeling is highly dependent on the glycosylated transmembrane protein extracellular matrix metalloproteinase (MMP) inducer (EMMPRIN). Previous studies indicate estradiol can increase EMMPRIN expression in uterine cells and promote subsequent induction of MMP production.

OBJECTIVE:

The aim of this study was to investigate the role of G protein-coupled receptor 30 (GPR30) stimulation on EMMPRIN microvesicle release in the human uterine epithelial cell line hTERT-EEC (EECs).

DESIGN:

We examined EMMPRIN release by human EECs in response to GPR30 stimulation by microvesicle isolation, Western blot, and immunocytochemistry. We employed a pharmacological approach using the GPR30-selective agonist G1 and the antagonist G15 to determine the receptor specificity of this response.

RESULTS:

We demonstrated GPR30 expression in EECs and release of EMMPRIN in microvesicles in response to stimulation of GPR30. G1, estradiol, and cholera toxin stimulated EMMPRIN release in microvesicles as detected by Western blot and immunocytochemistry, indicating that stimulation of GPR30 can induce EMMPRIN microvesicle release.

CONCLUSIONS:

These data indicate that EMMPRIN release in microvesicles can be mediated by stimulation of GPR30 in human EECs, suggesting that inappropriate stimulation or expression of this receptor may be significant in uterine pathology.

PMID:
23012390
PMCID:
PMC3513528
DOI:
10.1210/jc.2012-2098
[Indexed for MEDLINE]
Free PMC Article

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