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BMC Genomics. 2012 Sep 25;13:508. doi: 10.1186/1471-2164-13-508.

Genetic structure of community acquired methicillin-resistant Staphylococcus aureus USA300.

Author information

1
Scripps Genomic Medicine, Scripps Translational Science Institute, La Jolla, CA, USA.

Abstract

BACKGROUND:

Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) is a significant bacterial pathogen that poses considerable clinical and public health challenges. The majority of the CA-MRSA disease burden consists of skin and soft tissue infections (SSTI) not associated with significant morbidity; however, CA-MRSA also causes severe, invasive infections resulting in significant morbidity and mortality. The broad range of disease severity may be influenced by bacterial genetic variation.

RESULTS:

We sequenced the complete genomes of 36 CA-MRSA clinical isolates from the predominant North American community acquired clonal type USA300 (18 SSTI and 18 severe infection-associated isolates). While all 36 isolates shared remarkable genetic similarity, we found greater overall time-dependent sequence diversity among SSTI isolates. In addition, pathway analysis of non-synonymous variations revealed increased sequence diversity in the putative virulence genes of SSTI isolates.

CONCLUSIONS:

Here we report the first whole genome survey of diverse clinical isolates of the USA300 lineage and describe the evolution of the pathogen over time within a defined geographic area. The results demonstrate the close relatedness of clinically independent CA-MRSA isolates, which carry implications for understanding CA-MRSA epidemiology and combating its spread.

PMID:
23009684
PMCID:
PMC3598774
DOI:
10.1186/1471-2164-13-508
[Indexed for MEDLINE]
Free PMC Article

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