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[Human enterovirus 71 (EV71) resisted type I interferon induced antiviral effect].

[Article in Chinese]

Author information

1
Medical School, Anhui University of Science and Technology, Huainan 232001, China.

Abstract

OBJECTIVE:

To investigate human enterovirus 71 (EV71) resistance to type I interferon induced antiviral effect.

METHODS:

After type I interferons (alpha, beta) were incubated with HeLa cells, recombinant type I herpes simple virus (HSV-1) with green fluorescent protein (GFP) was inoculated onto the HeLa cells. HSV-1 proliferation was observed by GFP expression and PCR. After EV71 was inoculated onto HeLa cells incubated with the same quantity of interferon, proliferation of EV71 were detected by RT-PCR of 2A gene.

RESULTS:

Recombinant HSV-1 GFP expression and viral DNA replication obviously decreased in HeLa cells incubated with type I interferon (alpha, beta). However, EV71 effectively proliferated in the interferon irritated HeLa cell by RT-PCR.

CONCLUSION:

HeLa cell irritated by type I interferon (alpha, beta) produced antiviral substance that inhibits HSV-1 proliferation. EV71 resisted the antiviral substance induced by type I interferon and could significantly replicate in the HeLa cells.

PMID:
23002545
[Indexed for MEDLINE]
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