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Nucleic Acids Res. 2012 Nov;40(21):11155-62. doi: 10.1093/nar/gks840. Epub 2012 Sep 22.

Manipulation of gene expression in zebrafish using caged circular morpholino oligomers.

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1
State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, No. 38, Xueyuan Road, Beijing 100191, China.

Abstract

Morpholino oligomers (MOs) have been widely used to knock down specific genes in zebrafish, but their constitutive activities limit their experimental applications for studying a gene with multiple functions or within a gene network. We report herein a new design and synthesis of caged circular MOs (caged cMOs) with two ends linked by a photocleavable moiety. These caged cMOs were successfully used to photomodulate β-catenin-2 and no tail expression in zebrafish embryos.

PMID:
23002141
PMCID:
PMC3505977
DOI:
10.1093/nar/gks840
[Indexed for MEDLINE]
Free PMC Article
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