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Food Chem Toxicol. 2013 Jan;51:46-52. doi: 10.1016/j.fct.2012.09.011. Epub 2012 Sep 19.

Low-dose gamma irradiation of food protein increases its allergenicity in a chronic oral challenge.

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1
Unidade Acadêmica de Medicina Veterinária, CSTR, Universidade Federal de Campina Grande, Patos, Paraíba, Brazil. melo_vaz@ig.com.br

Abstract

Few chronic food protein models have described the relationship between allergenicity and the molecular structure of food protein after physical processing. The effect of γ-radiation on the structure of food protein was measured by fluorescence, circular dichroism and microcalorimetry. BALB/c mice were intraperitoneally sensitized and then given non-irradiated and irradiated Con-A by daily gavage for 28days. The tendency to form insoluble amorphous aggregates and partially unfolded species was observed after irradiation. The administration of non-irradiated and irradiated samples at low-dose significantly increased weight loss as well as plasma levels of eotaxin in animals repeatedly exposed to Con-A. Significant lymphocytic infiltrate filling completely the stroma of microvilli and tubular glands was observed in the small intestinal of the group given Con-A irradiated at a low dose. This phenotype was not observed in animals treated with Con-A irradiated at a high dose.

PMID:
23000443
DOI:
10.1016/j.fct.2012.09.011
[Indexed for MEDLINE]
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