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Structure. 2012 Nov 7;20(11):1905-17. doi: 10.1016/j.str.2012.08.025. Epub 2012 Sep 20.

The structure of the plk4 cryptic polo box reveals two tandem polo boxes required for centriole duplication.

Author information

1
Department of Biology, CB 3280, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.

Abstract

Centrioles are key microtubule polarity determinants. Centriole duplication is tightly controlled to prevent cells from developing multipolar spindles, a situation that promotes chromosomal instability. A conserved component in the duplication pathway is Plk4, a polo kinase family member that localizes to centrioles in M/G1. To limit centriole duplication, Plk4 levels are controlled through trans-autophosphorylation that primes ubiquitination. In contrast to Plks 1-3, Plk4 possesses a unique central region called the "cryptic polo box." Here, we present the crystal structure of this region at 2.3 Å resolution. Surprisingly, the structure reveals two tandem homodimerized polo boxes, PB1-PB2, that form a unique winged architecture. The full PB1-PB2 cassette is required for binding the centriolar protein Asterless as well as robust centriole targeting. Thus, with its C-terminal polo box (PB3), Plk4 has a triple polo box architecture that facilitates oligomerization, targeting, and promotes trans-autophosphorylation, limiting centriole duplication to once per cell cycle.

PMID:
23000383
PMCID:
PMC3496063
DOI:
10.1016/j.str.2012.08.025
[Indexed for MEDLINE]
Free PMC Article

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