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Diagn Microbiol Infect Dis. 2012 Dec;74(4):349-55. doi: 10.1016/j.diagmicrobio.2012.08.013. Epub 2012 Sep 19.

Rapid identification of pathogens from positive blood cultures by multiplex polymerase chain reaction using the FilmArray system.

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1
Department of Pediatrics, University of Utah, Salt Lake City, UT, USA. anne.blaschke@hsc.utah.edu

Abstract

Sepsis is a leading cause of death. Rapid and accurate identification of pathogens and antimicrobial resistance directly from blood culture could improve patient outcomes. The FilmArray® (FA; Idaho Technology, Salt Lake City, UT, USA) Blood Culture (BC) panel can identify >25 pathogens and 4 antibiotic resistance genes from positive blood cultures in 1 h. We compared a development version of the panel to conventional culture and susceptibility testing on 102 archived blood cultures from adults and children with bacteremia. Of 109 pathogens identified by culture, 95% were identified by FA. Among 111 prospectively collected blood cultures, the FA identified 84 (91%) of 92 pathogens covered by the panel. Among 25 Staphylococcus aureus and 21 Enterococcus species detected, FA identified all culture-proven methicillin-resistant S. aureus and vancomycin-resistant enterococci. The FA BC panel is an accurate method for the rapid identification of pathogens and resistance genes from blood culture.

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