Send to

Choose Destination
J Dairy Sci. 2012 Dec;95(12):6963-8. doi: 10.3168/jds.2012-5785. Epub 2012 Sep 19.

Technical note: Identification of Prototheca species from bovine milk samples by PCR-single strand conformation polymorphism.

Author information

Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche (CNR), Lodi, Italy.


We report the development of a PCR-single strand conformation polymorphism (SSCP) method to identify Prototheca spp. responsible for bovine mastitis: P. zopfii and P. blaschkeae. The method was set up using reference strains belonging to P. zopfii genotype 1, P. zopfii genotype 2, and P. blaschkeae as target species and P. stagnora, and P. ulmea as negative controls. The assay was applied on 50 isolates of Prototheca spp. isolated from bovine mastitic milk or bulk-tank milk samples, and all isolates were identified as P. zopfii genotype 2. We conclude that the described PCR-SSCP approach is accurate, inexpensive, and highly suitable for the identification of P. zopfii genotype 2 on field isolates but also directly on milk, if preceded by a specific DNA extraction method.

[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center